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KMID : 1024420180220030208
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Food Engineering Progress
2018 Volume.22 No. 3 p.208 ~ p.213
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The Preparation of Crystalline O-D-Mannopyranosyl-(1→4)-O-D-Glucopyranosyl-(1→4)-D-Mannopyranose (MGM) from Konjac Glucomannan Using Xylogone sphaerospora β-mannanase System and Candida guilliermondii Fermentation
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Park Gwi-Gun
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Abstract
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O-D-mannopyranosyl-(1→4)-O-D-glucopyranosyl-(1→4)-D-Mannopyranose (MGM) was prepared via the enzymatic hydrolysis of konjac glucomannan and the subsequent elimination of monosaccharides from the resultant hydrolysate using yeast. The enzyme system hydrolyzed konjac glucomannan and produced monosaccharides and MGM without other oligomers at the 48 h reaction. Konjac 20 g was hydrolyzed at 60°C and a pH 6.0 for 48 h with 200 mL crude enzyme solution from Xylogone sphaerospora. By eliminating monosaccharides from the hydrolysis products with yeast (Candida guilliermondii), 3.8 g of crystalline MGM was obtained, without the use of chromatographic techniques. After 48 h of yeast cultivation, the total sugar content fell from 5.2% to 3.7%, while the average degree of polymerization (D.P.) rose from 2.6 to 3.3.
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KEYWORD
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O-D-mannopyranosyl-(1→4)-O-D-glucopyranosyl-(1→4)-D-mannopyranose (MGM) , Xylogone sphaerospora , Candida guilliermondii , methylation method
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